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Mercury removal and recovery by immobilized

Meifang CHIEN, Ryo NAKAHATA, Tetsuya ONO, Keisuke MIYAUCHI, Ginro ENDO

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 192-197 doi: 10.1007/s11705-012-1284-3

摘要： From several mercury removing microorganisms, we selected MB1, which is non-pathogenic, broad-spectrum mercury resistant, mercuric ion reducing, heat tolerant, and spore-forming, as a useful bacterium for bioremediation of mercury pollution. In this study, mercury removal performance of the immobilized MB1 was investigated to develop safe, efficient and stable catalytic bio-agent for mercury bioremediation. The results showed that the alginate gel immobilized MB1 cells efficiently removed 80% of mercury from the solution containing 10 mg/L mercuric chloride within 24 h. These cells still had high activity of mercury removal even after mercuric ion loading was repeated for nine times. The analysis of mercury contents of the alginate beads with and without immobilized MB1 suggested that a large portion of reduced metallic mercury was trapped in the gel beads. It was concluded that the alginate gel immobilized MB1 cells have potential to remove and recover mercury from mercury-containing water.

关键词： mercury removal     immobilized bacteria     alginate gel     bioremediation    

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Immobilized

Pei MA, Dan ZHANG

《环境科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 498-508 doi: 10.1007/s11783-012-0429-4

摘要： To investigate the potential use of ( ) residue for Cd adsorption, poly alcohol Na alginate (PVA) was applied to immobilize it. The parameters including contact time, pH, adsorbent dosages, and coexisting metal ions were studied. The suitable pH for immobilized was 4–7 wider than that for raw (pH 6–7). In the presence of Pb concentration varying from 0 to 30 mg·L , the Cd adsorption ratios declined by 6.71% and 47.45% for immobilized and raw , respectively. While, with the coexisting ion Cu concentration varied from 0 to 30 mg·L , the Cd adsorption ratios declined by 12.97% and 50.56% for immobilized and raw , respectively. The Cd adsorption isotherms in single–metal and dual-metal solutions were analyzed by using Langmuir, Freundlich, and Dubinin–Radushkevich models. The Cd adsorption capacities ( ) in single-metal solution were 6.448 mg·L and 2.832 mg·L for immobilized and raw , respectively. The of immobilized were 1.850 mg Cd·g in Cd + Pb solution and 3.961 mg Cd·g in Cd + Cu solution, respectively. The Cd adsorption processes subjected to both adsorbents follow pseudo-second-order model. Mechanism study showed the functional group of was –OH, –NH, –CO, and PVA played an important role in metal adsorbing. Mining wastewater treatment test showed that PVA–SA-immobilized was effective in mixed pollutant treatment even for wastewater containing metal ions in very low concentration.

关键词： immobilization     Lentinus edodes residue     competitive adsorption     isotherm    

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Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第6期   页码 844-850 doi: 10.1007/s11783-013-0487-2

摘要： In this study, microorganisms (named B111) were immobilized on polyvinyl alcohol microspheres prepared by the inverse suspension crosslinked method. The biodegradation of bisphenol A (BPA) and 4-hydroxybenzaldehyde, a degradation product of BPA, by free and immobilized B111 was investigated. The BPA degradation studies were carried out at initial BPA concentrations ranging from 25 to 150 mg·L . The affinity constant K and maximum degradation rate were 98.3 mg·L and 19.7 mg·mg VSS·d for free B111, as well as 87.2 mg·L and 21.1 mg·mg VSS·d for immobilized B111, respectively. 16S rDNA gene sequence analyses confirmed that the dominant genera were and for BPA biodegradation in microorganisms B111.

关键词： bisphenol A     polyvinyl alcohol     immobilized microorganisms     4-hydroxybenzaldehyde    

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The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1356-4

摘要：

•Bacterial concentrations from eight stages were 104–105copies/m3.

关键词： Size distribution     Airborne bacteria     Biological diversity     Human pathogenic bacteria     Composting plants    

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Enhanced methane production during long-term UASB operation at high organic loads as enabled by the immobilized

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1505-4

摘要：

• Fungi enable the constant UASB operation even at OLR of 25.0 kg/(m3×d).

关键词： Anaerobic digestion     Fungi     Methane production     High OLR     Microbial community    

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of dilution rate on dynamic and steady-state biofilm characteristics during phenol biodegradation by immobilized

Veena Bangalore Rangappa, Vidya Shetty Kodialbail, Saidutta Malur Bharthaiyengar

《环境科学与工程前沿（英文）》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0863-9

摘要： Continuous pulsed plate bioreactor (PPBR) was used for phenol biodegradation. cells immobilized on granular activated carbon was used. Dynamic and steady state biofilm characteristics depend on dilution rate (DR). Lower DR favour phenol degradation and uniform, thick biofilm formation. Exo polymeric substance production in biofilm are favoured at lower dilution rates. Pulsed plate bioreactor (PPBR) is a biofilm reactor which has been proven to be very efficient in phenol biodegradation. The present paper reports the studies on the effect of dilution rate on the physical, chemical and morphological characteristics of biofilms formed by the cells of Pseudomonas desmolyticum on granular activated carbon (GAC) in PPBR during biodegradation of phenol. The percentage degradation of phenol decreased from 99% to 73% with an increase in dilution rate from 0.33 h?1 to 0.99 h?1 showing that residence time in the reactor governs the phenol removal efficiency rather than the external mass transfer limitations. Lower dilution rates favor higher production of biomass, extracellular polymeric substances (EPS) as well as the protein, carbohydrate and humic substances content of EPS. Increase in dilution rate leads to decrease in biofilm thickness, biofilm dry density, and attached dry biomass, transforming the biofilm from dense, smooth compact structure to a rough and patchy structure. Thus, the performance of PPBR in terms of dynamic and steady-state biofilm characteristics associated with phenol biodegradation is a strong function of dilution rate. Operation of PPBR at lower dilution rates is recommended for continuous biologic treatment of wastewaters for phenol removal.

关键词： Biofilm     Exopolymeric substances     Phenol     Dilution rate     Pulsed plate bioreactor    

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Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

《环境科学与工程前沿（英文）》 2023年 第18卷 第4期 doi: 10.1007/s11783-024-1808-3

摘要：

● The first study of electrochemically active magnetotactic bacteria.

关键词： Magnetotactic bacteria     Magnetospirillum magneticum     Magnetospirillum gryphiswaldense     Extracellular electron transfer     Microbial fuel cells    

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Regioselective acylation of pyridoxine catalyzed by immobilized lipase in ionic liquid

BAI Shu, REN Mengyuan, WANG Lele, SUN Yan

《化学科学与工程前沿（英文）》 2008年 第2卷 第3期   页码 301-307 doi: 10.1007/s11705-008-0060-x

摘要： The regioselective acylation of pyridoxine catalyzed by immobilized lipase () in 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF) has been investigated, and compared with that in acetonitrile (ACN). The acetylation of pyridoxine using acetic anhydride in [BMIM]PF gave comparable conversion of pyridoxine to 5-monoacetyl pyridoxine with considerably higher regioselectivity (93%–95%) than that in ACN (70%–73%). Among the tested parameters, water activity () and temperature have profound effects on the reaction performances in either [BMIM]PF or ACN. For the reaction in [BMIM]PF, higher temperature (50°C–55°C) and lower (<0.01) are preferable conditions to obtain better conversion and regioselectivity. Mass transfer limitation and intrinsic kinetic from the ionic nature of ionic liquids (ILs) may account for a different rate-temperature profile and a lower velocity at lower temperature in [BMIM]PF-mediated reaction. Moreover, consecutive batch reactions for enzyme reuse also show that lipase exhibited a much higher thermal stability and better reusability in [BMIM]PF than in ACN, which represents another advantage of ILs as an alternative to traditional solvents beyond green technology.

关键词： ACN     stability     1-butyl-3-methylimidazolium hexafluorophosphate     different rate-temperature     alternative    

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Organic and inorganic phosphorus uptake by bacteria in a plug-flow microcosm

Jinbo ZHAO, Xuehua LIU

《环境科学与工程前沿（英文）》 2013年 第7卷 第2期   页码 173-184 doi: 10.1007/s11783-013-0494-3

摘要： Phosphorus (P) is a vital nutrient for sustaining natural water productivity. Both particulate and dissolved forms of organic and inorganic P are potentially important sources of bioavailable P for primary and secondary producers. A microcosm system to imitate the bacterial community in Plym river sediment and pore water is described and bacterial uptake rates for inorganic and organic phosphorus are presented in this paper. The aim of this study was to investigate the uptake of two organic phosphorus compounds (phytic acid and D-glucose-6-phosphate) by freshwater bacteria. The bioreactors comprise glass columns packed with two types of small glass beads on which bacterial biofilm can develop. The glass beads with different porosity were introduced to simulate River SPM. The selected P compounds spiked into the inflow of the microcosm, and measured the step change of P concentration in the outflow to investigate the behavior of bacterial uptake of nutrients. The results showed that organic phosphorus was converted into inorganic phosphorus but the conversion rate depended on the type of phosphorus species. One experiment suggested that phytic acid (refractory) could displace phosphate from the biofilm surface; the other experiment showed that D-glucose-6-phosphate (labile) could be hydrolysed and utilized easily by the bacteria. The results also suggested that bacteria might break down the C-P bonds to utilize the carbon. Further experiments should investigate the effect of varying the C:N:P ratio in the microcosm system to determine which nutrient limits bacteria uptake.

关键词： organic phosphorus     bacteria     uptake    

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Visible light induces bacteria to produce superoxide for manganese oxidation

《环境科学与工程前沿（英文）》 2023年 第17卷 第2期 doi: 10.1007/s11783-023-1619-y

摘要：

● Term of manganese-oxidizing microorganisms should be reconsidered.

关键词： Mn(II) oxidation     Manganese-oxidizing bacteria     Reactive oxygen species     Mn(III/IV) oxides    

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Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

Shuang LIU, Yanwei HOU, Guoxin SUN

《环境科学与工程前沿（英文）》 2013年 第7卷 第2期   页码 191-199 doi: 10.1007/s11783-012-0470-3

摘要： The combination of two bacteria ( sp. PY1 and sp. PY2) and a fungus ( sp. PY3), isolated from contaminated soils near a coking plant, were investigated with respect to their capability to degrade pyrene and volatilize arsenic. The results showed that all strains could use pyrene and arsenic as carbon and energy sources in a basal salts medium (BSM), with the combined potential to degrade pyrene and volatilize arsenic. sp. PY1, sp. PY2 and sp. PY3 were isolated from the consortium and were shown to degrade pyrene and volatilize arsenic independently and in combination. Fungal-bacterial coculture has shown that the most effective removal of pyrene was 96.0% and volatilized arsenic was 84.1% after incubation in liquid medium after 9 days culture, while bioremediation ability was 87.2% in contaminated soil with 100 mg·kg pyrene. The highest level of arsenic volatilization amounted to 13.9% of the initial As concentration in contaminated soil after 63 days. Therefore, a synergistic degradation system is the most effective approach to degrade pyrene and remove arsenic in contaminated soil. These findings highlight the role of these strains in the bioremediation of environments contaminated with pyrene and arsenic.

关键词： pyrene     arsenic     bioremediation     bacteria     fungus    

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Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

《环境科学与工程前沿（英文）》 2007年 第1卷 第2期   页码 184-189 doi: 10.1007/s11783-007-0032-2

摘要： The purpose of this study was to investigate nitrifying bacteria and denitrifying bacteria isolated from aerobic granules. Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor (SBAR) and biodegradation of NH3--N was analyzed in the reactor. Bacteria were isolated and determined from aerobic granules using selected media. The growth properties and morphology of bacteria colonies were observed by controlling aerobic or anaerobic conditions in the culture medium. It was found that bacteria in aerobic granules were diverse and some of them were facultative aerobes. The diversity of bacteria in aerobic granules was a premise of simultaneous nitrification and denitrification.
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Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants

《化学科学与工程前沿（英文）》 2023年 第17卷 第7期   页码 867-879 doi: 10.1007/s11705-022-2277-5

摘要： Polydopamine-functionalized nanosilica was synthesized using an inexpensive and easily obtainable raw material, mild reaction conditions, and simple operation. Subsequently, a flexible spacer arm was introduced by using dialdehyde starch as a cross-linking agent to bind with laccase. A high loading amount (77.8 mg∙g‒1) and activity retention (75.5%) could be achieved under the optimum immobilization conditions. Thermodynamic parameters showed that the immobilized laccase had a lower thermal deactivation rate constant and longer half-life. The enhancement of thermodynamic parameters indicated that the immobilized laccase had better thermal stability than free laccase. The residual activity of immobilized laccase remained at about 50.0% after 30 days, which was 4.0 times that of free laccase. Immobilized laccase demonstrated excellent removal of phenolic pollutants (2,4-dichlorophenol, bisphenol A, phenol, and 4-chlorophenol) and perfect reusability with 70% removal efficiency retention for 2,4-dichlorophenol after seven cycles. These results suggested that immobilized laccase possessed great reusability, improved thermal stability, and excellent storage stability. Organic–inorganic nanomaterials have a good application prospect for laccase immobilization, and the immobilized laccase of this work may provide a practical application for the removal of phenolic pollutants.

关键词： polydopamine     pollutant removal     thermodynamic     phenolic pollutants     immobilized laccase    

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Airborne bacteria associated with particulate matter from a highly urbanised metropolis: A potential

《环境科学与工程前沿（英文）》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1552-5

摘要：

• The airborne bacteria of Mexico City are representative of urban environments.

关键词： Airborne bacteria     Urbanisation     PM10     Mexico City     Microbiome    

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Synergistic effects of sodium hypochlorite disinfection and iron-oxidizing bacteria on early corrosion

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1506-3

摘要：

• The early corrosion process in the cast iron pipes was investigated.

关键词： Cast iron pipe corrosion     Drinking water distribution systems     Chlorine disinfection     Iron-oxidizing bacteria     Coupling effects    

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标题 作者 时间 类型 操作

Mercury removal and recovery by immobilized

Meifang CHIEN, Ryo NAKAHATA, Tetsuya ONO, Keisuke MIYAUCHI, Ginro ENDO

期刊论文

Immobilized

Pei MA, Dan ZHANG

期刊论文

Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

期刊论文

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

期刊论文

Enhanced methane production during long-term UASB operation at high organic loads as enabled by the immobilized

期刊论文

of dilution rate on dynamic and steady-state biofilm characteristics during phenol biodegradation by immobilized

Veena Bangalore Rangappa, Vidya Shetty Kodialbail, Saidutta Malur Bharthaiyengar

期刊论文

Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

期刊论文

Regioselective acylation of pyridoxine catalyzed by immobilized lipase in ionic liquid

BAI Shu, REN Mengyuan, WANG Lele, SUN Yan

期刊论文

Organic and inorganic phosphorus uptake by bacteria in a plug-flow microcosm

Jinbo ZHAO, Xuehua LIU

期刊论文

Visible light induces bacteria to produce superoxide for manganese oxidation

期刊论文

Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

Shuang LIU, Yanwei HOU, Guoxin SUN

期刊论文

Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

期刊论文

Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants

期刊论文

Airborne bacteria associated with particulate matter from a highly urbanised metropolis: A potential

期刊论文

Synergistic effects of sodium hypochlorite disinfection and iron-oxidizing bacteria on early corrosion

期刊论文